Ozone Therapy for Pets: Uses, Evidence, and Safety

Medical ozone may be considered as a complementary option after veterinary evaluation. Evidence varies by condition, species, and administration method, and benefits are not guaranteed. This guide explains selected veterinary research, its limitations, and the questions to discuss before treatment.

Dr. Susset Diaz Castillo, DVM, PhD — Florida-licensed veterinarian, AHVMA member, and certified ozone practitioner. Meet Dr. Diaz. Verify license through Florida DBPR.

Mechanisms studied in ozone research

Ozone is a strong oxidant. Research examines oxidative-stress and antioxidant responses, as well as local antimicrobial effects. A proposed mechanism or a laboratory measurement does not, by itself, establish a clinical benefit for a sick dog or cat.

Administration methods described in veterinary literature

These methods are described for education, not as a menu of services offered by this practice. Routes are not interchangeable, and some are invasive. Dr. Diaz evaluates which method, if any, is appropriate after examining your pet.

Rectal Insufflation

An oxygen–ozone mixture administered through a rectal catheter. A small healthy-dog study assessed selected clinical and oxidative-stress measurements; it did not establish disease-treatment effectiveness.

Subcutaneous Injection

An invasive administration method described in veterinary literature. Suitability, risks, and expected benefits require individual veterinary assessment.

Major Autohemotherapy (MAH)

A procedure involving blood collection, controlled exposure to an oxygen–ozone mixture, and reinfusion. Findings from other administration methods cannot establish its safety or effectiveness.

Topical Ozonated Oils & Water

Ozonated preparations applied locally. Results with one preparation or procedure cannot be assumed to apply to every product, wound, or infection.

Ozonated Saline Irrigation

Ozonated liquids used for local irrigation in some protocols. Evidence must be assessed for the specific preparation and procedure.

Intra-Articular Injection

An invasive procedure involving a joint. Its inclusion here does not constitute a recommendation or confirmation that it is offered by this practice.

What veterinary studies show and their limitations

What veterinary studies have measured

A 2024 randomized cross-over study evaluated rectal insufflation in 10 healthy dogs. It reported changes in oxidative-stress measurements and selected clinical, blood, and biochemical variables, with measured variables remaining within reference ranges. It did not establish effectiveness for treating disease in sick pets or universal safety across species, administration methods, or protocols.

A separate 2024 study examined bacterial counts during dental procedures in 75 dogs. Its findings concern a specific preparation and procedure—not a general recommendation to replace indicated antibiotics. Dental cleaning is not offered by My Vet At Home®.

Limits of the evidence

Veterinary reviews describe differing protocols, small or uncontrolled studies, and incomplete outcome reporting. Findings from laboratory experiments, human studies, or other animal species cannot automatically establish treatment benefits in an individual dog or cat.

When veterinary evaluation is needed

Pain, wounds, skin or ear disease, gastrointestinal concerns, and other symptoms require diagnosis and appropriate treatment. These concerns should not be presented as a list of conditions proven to benefit from ozone.

Safety and veterinary screening

Ozone treatment is not risk-free. Suitability and risks depend on the pet's health, the administration method, and the treatment protocol. Small studies in healthy dogs cannot establish universal safety or long-term benefit. Dr. Diaz discusses the available evidence, uncertainties, alternatives, and monitoring before recommending treatment.

Veterinary screening includes your pet’s medical history, examination findings, current medications, and testing when indicated. Suitability cannot be determined from a general online checklist. Do not attempt treatment yourself or expose your pet to ozone gas for inhalation.

What ozone therapy does not replace

Ozone must not replace or delay indicated antibiotics, pain relief, cancer care, dental treatment, surgery, or emergency care. Antimicrobial findings in a laboratory or a specific dental study do not establish that ozone can replace antibiotics for other infections.

What a treatment course looks like

There is no guaranteed number of sessions or recovery timeline. If treatment is recommended, Dr. Diaz explains the proposed schedule, goals, costs, and monitoring. Your pet’s response is reassessed to decide whether to continue, change, or stop treatment.

Frequently asked questions

What exactly is medical ozone, and how is it different from the ozone in smog?

Medical ozone procedures use oxygen–ozone mixtures or ozonated preparations. Ozone is the same O₃ molecule found in the atmosphere; preparations, concentrations, and administration methods differ. This does not make ozone risk-free. Ozone gas must not be inhaled.

Is ozone therapy painful or stressful for my pet?

Comfort and sedation needs depend on the method, your pet, and the condition being treated. Some methods are invasive. Dr. Diaz explains the procedure and any pain relief, sedation, or monitoring that may be needed.

How many ozone therapy sessions does my pet need before we see results?

There is no guaranteed number of sessions or recovery timeline. If treatment is recommended, Dr. Diaz explains the proposed schedule, goals, costs, and monitoring. Your pet’s response is reassessed to decide whether to continue, change, or stop treatment.

Is ozone therapy safe for senior pets or pets with chronic disease?

Age alone does not determine suitability. Research in healthy dogs does not establish safety for every senior or chronically ill pet. Dr. Diaz reviews the diagnosis, medications, proposed method, risks, and alternatives before making a recommendation.

Can ozone therapy replace antibiotics, NSAIDs, or chemotherapy?

Ozone must not replace or delay indicated antibiotics, pain relief, cancer care, dental treatment, surgery, or emergency care. Laboratory antimicrobial findings or a specific dental study do not establish that it can replace antibiotics for other infections.

What does ozone therapy cost?

Care begins with an All-Included Visit and veterinary evaluation. See our current care packages and inclusions. Any proposed treatment costs are explained before proceeding. Insurance reimbursement depends on your policy; confirm coverage with your insurer.

How do I find a qualified veterinary ozone practitioner?

Look for a licensed veterinarian with relevant training, documented equipment and dosing protocols, and an individualized treatment plan. Ask about the evidence, risks, alternatives, costs, monitoring, and criteria for stopping treatment. Training and professional membership do not establish treatment effectiveness.

Is ozone therapy FDA-approved?

Research publications and practitioner training do not establish FDA approval. FDA regulations describe ozone as a toxic gas with “no known useful medical application in specific, adjunctive, or preventive therapy.” Extra-label drug prescribing concerns approved human or animal drugs; it is not blanket authorization for ozone procedures. Ask about the regulatory status, evidence, and risks of the specific product and method proposed.

Can I do ozone therapy at home myself?

No. Do not attempt treatment yourself or expose your pet to ozone gas for inhalation. Veterinary screening, appropriate equipment, and monitoring are necessary; administration methods may be invasive and carry risks.

See current care packages and inclusions.

Veterinary references and their limitations

  1. de Oliveira PL, Mendonça MO, Gonçalves GS, et al. Ozone therapy by rectal insufflation in dogs: safety and oxidative stress — a randomized cross-over study. Veterinary Research Communications, 2024. Study of 10 healthy dogs; not proof of disease-treatment effectiveness or universal safety.
  2. Melanie P, Niola C, Plataroti I, et al. Use of Ozone in Veterinary Dentistry as an Alternative to Conventional Antibiotics and Antiseptics. Veterinary Sciences, 2024. Study of bacterial counts during dental procedures in 75 dogs; not a general recommendation to replace indicated antibiotics. Dental cleaning is not offered by this practice.
  3. Orlandin JR, Machado LC, Ambrósio CE, Travagli V. Ozone and its derivatives in veterinary medicine: A careful appraisal. Veterinary and Animal Science, 2021. Review discussing incomplete data, small or uncontrolled studies, and lack of standardized protocols.
  4. Sciorsci RL, Lillo E, Occhiogrosso L, Rizzo A. Ozone therapy in veterinary medicine: A review. Research in Veterinary Science, 2020. Review of mechanisms and applications across species; not a guarantee of clinical benefit in an individual dog or cat.

Regulatory sources

  • FDA ozone regulation — 21 CFR 801.415
  • FDA explanation of extra-label drug use

Care begins with an All-Included Visit. Dr. Diaz evaluates your pet and may recommend ozone as part of an individualized care plan. Ozone is not booked as a standalone treatment. Serving select areas of Miami-Dade County and select areas of Broward County. Book online to check your ZIP and available visit times. For questions, call (786) 516-4731.

See our ozone therapy service.

VestaSoul — My Vet At Home® is a mobile veterinary house-call practice serving select areas of Miami-Dade County and select areas of Broward County.